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KMID : 0376219690060030415
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Chonnam Medical Journal
1969 Volume.6 No. 3 p.415 ~ p.435
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Histological and Histochemical Studies of the Rat Epididymis.
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Abstract
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In an attempt to correlate known function of the rat epididymis was composed partly of ductuli efferentes and largely of ductus epididymis. The epithelium of the canal consisted of predominantly, the ciliated columnar cells, occasional nonciliated club-shaped cells attached to the basement membrane with its slender cytoplasm and basal cells found in the basal portion of the epithelium.
The epididymal canal forming the body was similar in cellular components to those of the head except replacing club-shaped cells with goblet type cells in which saliva resistant PAS positive granules were found mainly in the supranuclear region of the cell.
Tail portion of the epididymis showed the canal having thin wall and dilated lumen and wide intersitium containing blood vessels and various cells. In the thin epithelial layer PAS
positive goblet cells were increased in number.
2. Alkaline phosphatase activity of the epididymal canal was negative at the luminal surface of the epithelium and was moderate at the basement membrane and smooth muscle around the basement membrane, although blood vessels showed marked activity.
3. Acid phosphatase activity was seen as the granules in the epithelial cells of the canal. Most prominent activity was found in the canal of the body segment and less marked in the head and the tail portion of the epididymis.
In the epitbelium of epididymal canal of the tail, there was a cell type in which difuse or fine granular activity was found, besides the ordinary cells in which the granules active to the enzyme were aggregated.
4. The activicy of ATPase, ADPase, and AMPase was noted in bisement membrane, smooth muscle and blood vessels.
These enzyme activities were most marked at the luminal surface of the canal in the body region of the epididymis, in which even more pronounced activity was present after staining for AMPase.
Nonciliated blub-shaped or goblet type cells in the head and the tail showed striking activities of these three enzymes on the tissue fixed in formol-calcium solution.
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KEYWORD
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